Journal: Advanced Science

Article Title: Inflammatory Microenvironment‐Responsive Hydrogels Enclosed with Quorum Sensing Inhibitor for Treating Post‐Traumatic Osteomyelitis

doi: 10.1002/advs.202307969

Figure Lengend Snippet: Characterizations of hydrogels. a) Photos of the double‐syringe system and the injectability of the HBPL‐gel. b,c) SEM images of the Gel in PBS solution and H 2 O 2 solution. d) Storage modulus (G′) and loss modulus (G″) of the Gel at 1hz and 1% dynamic strain. e) Drug release percentage in PBS (pH 7.4), 1 m m H 2 O 2 and PBS (pH 5.5). f) Different types of ROS. g–j) g)·OH, h)·O 2 − , i) DPPH, and j) H 2 O 2 clearance of hydrogels. k) Photographs of Gel in different solutions. l) Fluorescence microscope images of H 2 O 2 ‐stimulated RAW264.7 with different treatments. Data are presented as mean ± SD. ( n = 3–5 per group, ns: no significance, *** p < 0.001).

Article Snippet: The scavenging abilities of ·OH, ·O 2 − , DPPH and H 2 O 2 were detected by the Hydroxyl Free Radical Scavenging Capacity Assay Kit (Elabscience, USA), Inhibition and Production of Superoxide Anionic Colorimetric Assay Kit (Elabscience, USA), DPPH Free Radical Scavenging Capacity Assay Kit (Solarbio, China), and Hydrogen Peroxide Colorimetric Assay Kit (Elabscience, USA) respectively, according to the manufacturer's instructions.

Techniques: Fluorescence, Microscopy

Journal: Antioxidants

Article Title: Physiological and Clinical Aspects of Bioactive Peptides from Marine Animals

doi: 10.3390/antiox11051021

Figure Lengend Snippet: Antioxidant effects of bioactive peptides from marine animals—in vitro studies.

Article Snippet: (i) EDIVCW (ii) MGPVW (iii) YWDAY , (i) 763.82 (ii) 660.75 (iii) 793.75 , Monkfish ( Lophius litulon ) , HepG2 , DPPH • , • OH, H 2 O 2, and O 2 •− scavenging activity, ROS levels, lipid peroxidation inhibition assay, Q-TOF MS, MTT assay , - Peptide (i) showed DPPH • scavenging activity of 83% - Peptide (iii) showed • OH and O 2 •− scavenging activity of 85% and 80%, respectively - Peptide (iii) showed SOD, GPx, and CAT levels of 175, 45, and 22.5 U/mg protein, respectively , [ ] .

Techniques: TBARS Assay, High Performance Liquid Chromatography, Activity Assay, Inhibition, MTT Assay, Hemolysis Assay, FRAP Assay, Fluorescence In Situ Hybridization, Filtration, Chromatography, Gel Permeation Chromatography, Antioxidant Activity Assay, Plasmid Preparation, Staining, Purification, Cytotoxicity Assay, Concentration Assay, Viability Assay, Membrane, CCK-8 Assay